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Rodent nutritional model of non-alcoholic steatohepatitis: species, strain and sex difference studies

10.3389/fphar.2020.585612 85 MaedaS.MatsuiT.YamagishiS.-i

Lentivirus preparation and transduction To prepare lentivirus particles, 4 10 5 HEK293T cells were seeded into six-well plates and transfected the next day with 0.66 g expression plasmid, 0.44 g pCMV-dR8.91 (gag/pol) and 0.88 g pMD.G vesicular stomatitis virus G glycoprotein using 8 l FuGENE HD transfection reagent (Promega)