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Pike LJ, Han X, Chung KN, Gross RW

Engineering and characterization of a superfolder green fluorescent protein

72 Bone loss is influenced by initial body weight, age, sex, physical activity, extent of energy restriction and protein intake, and rate of weight reduction, with older individuals and females experiencing greater bone loss

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The position and identity of the residues essential for catalytic activity within the C-terminal region of these related enzymes are highly conserved in prokaryotes and eukaryotes despite other significant differences in sequence (23)