Bd growth was assessed by taking 3 randomly chosen images at 4x magnification fields of view (FOV) per well under red fluorescence, and calculating the total area occupied by the red fluorescing Bd cells using image J relative to the control average (36)
Sterilization methods, such as gamma radiation or dry heat, should be used to maintain the physical and chemical properties of the NPs
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Then, the 1% silk fibroin solution mixed with GLP-1RAs was dropped into 5 ml of acetone solution, vortexed, centrifuged, and the NPs precipitate was resuspended with double-distilled water to obtain the GLP-1RAs@NPs solution