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The reaction mixture was incubated at 30 C and the consumption of NADH was monitored continuously for 60 min with a TECAN M200 plate reader by measuring the absorbance at 340 nm

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Once mixed with liquid, refrigerate and use within a week, or freeze it for longer, avoiding repeated freezing and thawing

Preparation of Solution-2: Transferred about 0.7 mL of Triethylamine into a 100 mL volumetric flask