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Andrographolide is the major active component of AP, exhibits diverse pharmacological activities, including anti-inflammatory, antioxidant, and antidiabetic activities ( In vitro and in vivo studies demonstrated that Rg1 down-regulated MDA and ROS, up-regulated SOD, increased the expression of Bcl-2 protein, decreased the activity of proteins such as Bax, cleaved cystathionase-3, and cleaved cystathionase-9, and effectively inhibited the oxidative stress response and reduced apoptosis in rats with DN ( 3.3.2 Steroidal saponins Diosgenin (DIO) is a plant sterol saponin, since time immemorial, DIO and its derivatives, which are obtained from the rhizomes of Dioscorea (popularly known as Shanyao in Chinese) are utilized in traditional Chinese medications ( 3.3.3 Flavonoid Formononetin (FMN) is one of the major isoflavonoids isolated from Astragalus, which has been found to have antioxidant activity in some studies ( in vitro experiments and has mitochondrial protective and anti-apoptotic effects (114)

Additionally, GCH1 promotes CoQH2 production and phospholipids (PLs) containing two PUFA tails, which provide ferroptosis defense
making it one of the most versatile and widely researched healing compounds available
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