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Further purification was done by means of analytical HPLC on a 250 4.6 mm, 4 m, 90 A, Jupiter Proteo column (Phenomenex)

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Specifically, NPY and Y1 receptor knock-out mice showed decreased eosinophils in the bronchoalveolar lavage fluid, as well as decreased circulating immunoglobulin E (IgE) levels, compared to wild-type mice under allergic conditions [178]

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However, these drugs work best when combined with lifestyle changes, including diet and exercise