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The expression and location of GLP-1R in testicular tissues and in four testicular cell lines (spermatogonia, spermatocytes, Leydig cells, and Sertoli cells) were detected

Prepare the injection and hold the syringe at 90 degrees to the skin

The release of cytokines and autoantibodies are reported in antithyroid drugs-treated patients and/or laboratory animals.56,59 It has been shown that cytokine-mediated liver injury could has a critical role in methimazole-treated mice.56 Furthermore, some cases of PTU-induced hepatic damage are reported in which autoantibodies are demonstrated.60 Lymphocyte sensitization in a patient with neonatal liver injury probably by placental transfer of PTU has been reported.61 All these reports are in line with the hypothesis that patient immune system might play a role in the pathogenesis of hepatic injury induced by antithyroid medications

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Experimental protocols were reviewed and approved by the Institutional and Animal Care Use Committee (IACUC) of Baylor College of Medicine