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RTqPCR Total RNA was isolated using the RNeasy Mini Kit (Qiagen, 74104) and reverse-transcribed with a high-capacity RNA-to-cDNA kit (Vazyme, R323-01)

Melittin exerts an antitumor effect on non-small cell lung cancer cells

Cell Signaling technology, Beverly, MA, USA) for overnight at 4 C

A homology analysis of the active site, oxyanion hole, CAS, PAS, acyl pocket, and active-site entrance residues revealed clear differences among available AChE coding sequences

Each peptide below has been evaluated based on its mechanism of action, clinical evidence specifically relevant to menopausal women, practical considerations like dosing and side effects, and how well it integrates into a comprehensive protocol