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Subsequently, fertilized oocytes from each group were transferred individually to embryo culture droplets as SOF medium supplemented with 0.5 mM sodium citrate, 1 mM sodium pyruvate, 1% non-essential amino acids, 2% essential amino acids, 2 mM Gluta MAX , 2.8 mM myo-inositol and 4 mg/ml bovine serum albumin covered by mineral oil in a 35 mm sterile dish, followed by embryo culture in a CO 2 incubator (38.5C, 5%O 2 and 5%CO 2 ) (71)

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