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Binding of AIM to Fc/J was performed with the same protocol in the presence of 2.0 mM of various divalent cation chlorides (CaCl 2 , MgCl 2 , MnCl 2 , CoCl 2 , NiCl 2 , ZnCl 2 ) in triplicate wells in a single experiment

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Then, 1% thiobarbituric acid was added to the clarified homogenate and incubated at 90 C for 1 h, followed by addition of 250 L of n-butanol

Cellular uptake studies were performed in cellular environments with different oxygen concentrations in the cell lines 143B and K7M2