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SuperScript Reverse Transcriptase (Thermoscientific) was used to synthesize first-strand cDNA following the manufacturers instructions 44

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It was noticed that the antibacterial activity increases with the increase in the number of sulfur, suggesting that antibacterial activity is mediated by formation of disulfide bonds between the compounds and bacterial protein, mainly enzymes

However, indirect effectssuch as improved renal function or blood pressure reductionmay modulate anticoagulant efficacy over weeks to months, requiring periodic assessment rather than immediate dose adjustment