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RPMI-1640 medium with 10% foetal bovine serum (FBS) (F7524, Sigma Aldrich) was added and mixed vigorously for 20 seconds to stop the reaction

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Apoptotic populations were identified using a standard Annexin V/PI quadrant gating strategy: Annexin V /PI cells were considered viable cells, Annexin V + /PI cells were classified as early apoptotic cells, Annexin V + /PI + cells as late apoptotic cells, and Annexin V /PI + cells as necrotic cells