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Cellular senescence was assessed by SA--galactosidase staining (SA--gal staining) using senescence -galactosidase Staining Kit (Cell Signaling Technology, Beverly, MA, United States) according to the manufacturers instructions

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His6- and GST-tagged protein samples were cleaved using TEV protease, followed by repeated purification on an Ni-NTA column

Figure 3: The average number of crossing into the target quadrant in each of the four different experimental groups included in the first part of the study

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