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L-carnitine is often preferred for its direct role in fat metabolism
Tobacco BY-2 cells (RIKEN BRC through the National BioResource Project) were cultured in modified Linsmaier and Skoog medium (pH 5.5), which contained Murashige and Skoog Plant Salt Mixture (Wako), 30 g l 1 sucrose, 0.2 g l 1 KH 2 PO 4 , 100 mg l 1 myo-inositol, 1 mg l 1 thiamine-HCl, 0.2 mg l 1 2,4-dichlorophenoxyacetic acid and NaOH, in the dark at 25 C with agitation at 130 rpm
Early research on ferroptosis-characteristic cell death is dated back to the 1950s and 1960s, led by Harry Eagle who demonstrated that cysteine depletion reduced glutathione and led to cell death, whereas cysteine synthesis protected cell death by restoring glutathione [94,95,96]
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